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Test Code ACTHM Adrenocorticotropic Hormone (ACTH), Plasma

Additional Codes







Reporting Name

Adrenocorticotropic Hormone, P

Useful For

Determining the cause of hypercortisolism and hypocortisolism

Method Name

Electrochemiluminescence Immunoassay

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Plasma EDTA

Specimen Required

Patient Preparation: 12 hours before this blood test do not take multivitamins or dietary supplements containing biotin or vitamin B7, which are commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube: Ice-cooled, lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Morning (6 a.m.-10:30 a.m.) specimen is desirable.

2. Collect with a pre-chilled EDTA tube and transport to the laboratory on ice.

3. Spin down in a refrigerated centrifuge within 2 hours and immediately separate plasma from cells.

4. Immediately freeze plasma.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen (preferred) 28 days
  Refrigerated  3 hours
  Ambient  2 hours

Reject Due To


Mild OK; Gross reject


Mild OK; Gross OK






Reference Values

7.2-63 pg/mL (a.m. draws)

No established reference values for p.m. draws

Pediatric reference values are the same as adults, as confirmed by peer reviewed literature.


Petersen KE: ACTH in normal children and children with pituitary and adrenal diseases. I. Measurement in plasma by radioimmunoassay-basal values. Acta Paediatr Scand 1981;70:341-345

Day(s) and Time(s) Performed

Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
ACTH Adrenocorticotropic Hormone, P 2141-0


Result ID Test Result Name Result LOINC Value
ACTH Adrenocorticotropic Hormone, P 2141-0

Analytic Time

Same day/1 day

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

General Request Form (T239) (

Oncology Test Request Form (T729) (