Sign in →

Test Code CIB Cutaneous Direct Immunofluorescence Assay (IFA), Biopsy


Necessary Information


Location of biopsy site; whether biopsy was obtained from sun-exposed verses unexposed skin; whether it is from perilesional, involved, or uninvolved skin; and date of collection are required.

 

Pathology Report/Clinical Notes (include a brief history, pertinent laboratory results, suspected diagnosis and reason for referral).



Specimen Required


Two or more biopsies from same site and sent in 1 specimen vial will be processed as 1 specimen. Two or more biopsies from different sites require separate orders and separate specimen vials.

 

Transport Medium Method

Supplies: Michel's Transport Media for Immunofluorescent Testing on Tissue (T321)

Specimen Type: Tissue

Sources: Skin, oral (oropharyngeal), nasal, genital, esophagus, conjunctiva larynx, epiglottis, or bronchus mucosa 

Container/Tube: Transport medium (Michel's-T321 also called Zeus media)

Specimen Volume: 2-8 mm punch specimen, intact or bisected; excisional biopsy specimen intact or bisected

Collection Instructions:

1. Collect biopsy of uninvolved or involved skin.

2. Immediately place specimen into a labeled vial of transport medium and seal tightly.

Specimen Stability Information: Ambient (preferred)

 

Snap-Frozen Method

Specimen Type: Tissue

Sources: Skin, oral (oropharyngeal), nasal, genital, esophagus, conjunctiva larynx, epiglottis, or bronchus mucosa 

Container/Tube: Plastic vial

Specimen Volume: 2-8 mm punch specimen, intact or bisected; excisional biopsy specimen, intact or bisected

Collection Instructions:

1. Collect biopsy of uninvolved or involved skin.

2. Immediately place specimen into liquid nitrogen and allow to freeze thoroughly (do not allow specimen to desiccate). If liquid nitrogen is not available, specimen may be frozen by placing it on a small square of aluminum foil on a block of dry ice. Liquid nitrogen is preferred.

3. Immediately wrap specimen carefully in aluminum foil. At no time should the specimen be allowed to thaw.

4. Place the wrapped specimen into the prelabeled plastic vial and seal tightly.


Forms

If not ordering electronically, complete, print, and send a Pathology Test Request Form (T246) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/pathology-request-form.pdf).

Useful For

Confirming a diagnosis of bullous pemphigoid, cicatricial pemphigoid, pemphigoid gestationis and other variants of pemphigoid, all types of pemphigus, including paraneoplastic pemphigus (paraneoplastic multiorgan syndrome), dermatitis herpetiformis, linear IgA bullous dermatosis, chronic bullous disease of childhood, epidermolysis bullosa acquisita, porphyria cutanea tarda, bullous eruption of lupus erythematosus, and atypical or mixed forms of bullous disease, systemic lupus erythematosus, cutaneous lupus erythematosus, or other variants, vasculitis, lichen planus, and other inflammatory diseases

 

This test is not useful for diagnosis of malignancies involving the skin.

Method Name

Direct Immunofluorescence Staining of Cryostat-Prepared Skin Biopsy Sections for IgG, IgM, IgA, C3, and Fibrinogen Deposition

Reporting Name

Cutaneous Direct IFA, Biopsy

Specimen Type

Varies

Specimen Minimum Volume

Entire specimen

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Frozen 
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Formalin-fixed specimen

Reference Values

Report includes description and interpretation of staining patterns.

Day(s) and Time(s) Performed

Monday through Friday; 7:30 a.m.-5 p.m.

Analytic Time

2 days (not reported on Saturday or Sunday)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88346

88350 x 4

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CIB Cutaneous Direct IFA, Biopsy In Process

 

Result ID Test Result Name Result LOINC Value
71145 Interpretation In Process
71146 Participated in the Interpretation No LOINC Needed
71147 Report electronically signed by In Process
71610 Addendum In Process
71855 Case Number In Process