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Test Code ERPR Estrogen/Progesterone Receptor, Semi-Quantitative Immunohistochemistry, Manual


Advisory Information


This test is for prognostic purposes only.

 

For diagnostic purposes, order PATHC / Pathology Consultation and then request the stains.

 

Estrogen/progesterone receptor testing is not appropriate and not performed for cases of lobular carcinoma in situ.



Shipping Instructions


Attach the green pathology address label included in the kit to the outside of the transport container.



Necessary Information


1. Include accompanying pathology report stating the final diagnosis. If not available, a preliminary diagnosis is acceptable.

2. Information regarding fixative used, time to fixation, and duration of fixation is required.



Specimen Required


Specimen Type: Breast carcinoma

Preferred: A paraffin-embedded tissue block containing in-situ, invasive or metastatic breast carcinoma tissue that has been fixed in 10% neutral buffered formalin within 1 hour from surgical collection time and for a total of 6 to 72 hours and shipped at ambient temperature

Acceptable: 3 unstained sections, containing carcinoma, on charged slides cut at 4 microns <1 month ago and shipped at ambient temperature

 

Supplies: Pathology Packaging Kit (T554)

Specimen Type: Non-breast carcinoma

Preferred: A paraffin-embedded tissue block containing carcinoma tissue that has been fixed in 10% neutral buffered formalin and shipped at ambient temperature

Acceptable: 3 unstained sections, containing carcinoma, on charged slides cut at 4 microns <1 month ago and shipped at ambient temperature

Container/Tube: Pathology Packaging Kit (T554)

Collection Instructions: Submit paraffin-embedded carcinoma tissue.

Additional Information:

1. When ordering ERPR / Estrogen/Progesterone Receptor, Semi-Quantitative Immunohistochemistry, Manual, the following questions, as stated on the order form or presented electronically, must be answered:

a. "Was specimen fixed in 10% neutral buffered formalin within 1 hour from surgical collection time? Yes, No, or Unknown."

b. "Has specimen been fixed in 10% neutral buffered formalin for 6 to 72 hours? Yes, No, or Unknown."

2. According to the College of American Pathologists (CAP)/American Society of Clinical Oncology (ASCO) guidelines, estrogen/progesterone receptor protein immunohistochemical test results are only valid for nondecalcified, paraffin-embedded specimens fixed in 10% neutral buffered formalin within 1 hour from surgical collection time and for a total time of 6 to 72 hours. Delay to fixation, under-or overfixation may affect these results.

3. Paraffin blocks will be returned with final report.


Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Pathology Test Request Form (T246) (http://www.mayomedicallaboratories.com/it-mmfiles/pathology-request-form.pdf)

Oncology Test Request Form (T729) (http://www.mayomedicallaboratories.com/it-mmfiles/oncology-request-form.pdf)

Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request Form (T763) (http://www.mayomedicallaboratories.com/it-mmfiles/ihc-and-ish-stain-request-form-mc0767-09a.pdf)

Useful For

Guiding decisions on hormonal therapy in patients with breast carcinomas

Method Name

Semi-Quantitative Immunohistochemistry

Reporting Name

ER/PR Semi Quant IHC Manual

Specimen Type

Special

Specimen Stability Information

Specimen Type Temperature Time
Special Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

Reference Values

Negative: <1% reactive cells 

Positive: >1% reactive cells

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

2 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

CPT Code Information

88360 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ERPR ER/PR Semi Quant IHC Manual In Process

 

Result ID Test Result Name Result LOINC Value
70965 Interpretation In Process
70966 Participated in the Interpretation In Process
70967 Report electronically signed by 19139-5
70969 Material Received 81178-6
MA002 Fixed in 10% NB formalin w/in 1 hr In Process
MA003 Fixed in 10% NB formalin 6-72 hrs In Process
MA004 Tumor type In Process
MA005 Tumor classification In Process
71620 Disclaimer 62364-5
71834 Case Number In Process