Sign in →

Test Code HCVRTM Hepatitis C Virus (HCV) RNA Detection and Quantification by Real-Time Reverse Transcription-PCR (RT-PCR), Serum

Additional Codes

 

MAYO Test ID

HCVQN

EPIC Test ID

LAB887

 


Advisory Information


For detection and quantification of HCV RNA in serum before, during, and after antiviral therapy for chronic hepatitis C.



Shipping Instructions


1. Freeze serum immediately, and ship specimen frozen on dry ice only.

2. If shipment will be delayed for more 24 hours, freeze serum at -20 to -80° C (up to 84 days) until shipment on dry ice.



Specimen Required


Supplies: Aliquot Tube, 5 mL (T465)

Collection Container/Tube: Serum gel

Submission Container/Tube: Polypropylene vial (T465)

Specimen Volume: 1.5 mL

Collection Instructions: Spin down and separate serum from cells within 24 hours of collection (unspun samples should be stored at 2 to 25° C).


Useful For

Detection of acute hepatitis C virus (HCV) infection before the appearance of HCV antibodies in serum (ie, <2 months from exposure)

 

Detection and confirmation of chronic HCV infection

 

Quantification of HCV RNA in serum of patients with chronic HCV infection (HCV antibody-positive)

 

Monitoring disease progression in chronic HCV infection and response to antiviral therapy

 

Determining cure and detection of relapse after completion of antiviral therapy

Testing Algorithm

The following algorithms are available in Special Instructions:
-Hepatitis C: Testing Algorithm for Screening and Diagnosis
-Chronic Hepatitis C Treatment and Monitoring Algorithm: Interferon-Free Combination Therapy

Method Name

Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Reporting Name

HCV RNA Detect/Quant, S

Specimen Type

Serum SST

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum SST Frozen (preferred) 84 days
  Refrigerated  6 days

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

Red top tube or tube with any anticoagulant

Reference Values

Undetected

Day(s) and Time(s) Performed

Monday through Saturday; 7 a.m.-4 p.m.

Analytic Time

Monday through Thursday:, 1 day; Friday, and Saturday:, 3 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87522

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HCVQN HCV RNA Detect/Quant, S 11011-4

 

Result ID Test Result Name Result LOINC Value
97291 HCV RNA Detect/Quant, S 11011-4