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Test Code HIV1RM HIV-1 RNA Detection and Quantification, Plasma

Additional Codes








Useful For

Diagnosis of HIV-1 infection in individuals with acute or early HIV-1 infection


Diagnosis of HIV-1 infection in infants of <18 months of age born to HIV-1-infected mothers


Quantifying plasma HIV-1 RNA levels (viral load) in HIV-1-infected individuals:

-Before initiating anti-HIV-1 drug therapy (baseline viral load)

-Who may have developed HIV-1 drug resistance while on anti-HIV-1 therapy

-Who may be noncompliant with anti-HIV-1 drug therapy


Monitoring HIV-1 disease progression while on or off antiretroviral drug therapy

Testing Algorithm

The following algorithms are available in Special Instructions:

-HIV Testing Algorithm (Fourth Generation Screening Assay) Including Follow-up of Reactive HIV Rapid Serologic Test Results

-HIV Treatment Monitoring Algorithm

Method Name

Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Reporting Name

HIV-1 RNA Detect / Quant, P

Specimen Type

Plasma EDTA

Specimen Required

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.5 mL

Collection Instructions:

1. Spin down and remove plasma from cells within 6 hours of draw.

2. Freeze plasma specimen immediately, and ship specimen frozen on dry ice. Specimen can be sent refrigerate up to 5 days.

3. If shipment will be delayed for >5 days, freeze plasma specimen at -70° C (up to 35 days) until shipment on dry ice.

Additional Information: This test can be used for detection and diagnosis of HIV-1 infections, including in children <18 months of age when serologic tests are not useful (due to presence of maternal HIV antibodies).

Specimen Minimum Volume

1.2 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen (preferred) 35 days
  Refrigerated  5 days

Reject Due To


Mild OK; Gross OK


Mild OK; Gross OK




Green-top (heparin) tube

Reference Values


Day(s) and Time(s) Performed

Monday through Saturday; 7 a.m.-4 p.m.

Analytic Time

Monday through Thursday, 1 day; Friday and Saturday, 3 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
HIVDQ HIV-1 RNA Detect / Quant, P 70241-5


Result ID Test Result Name Result LOINC Value
63436 HIV-1 RNA Detect/Quant, P 70241-5


If not ordering electronically, complete, print, and send a General Request Form (T239) with the specimen (