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Test Code HOMPCR Mycoplasma hominis, Molecular Detection, PCR

Additional Codes

 

MAYO Test ID

MHRP

EPIC Test ID

LAB2913

 

Reporting Name

Mycoplasma hominis PCR

Useful For

Rapid, sensitive, and specific identification of Mycoplasma hominis from synovial fluid, genitourinary, reproductive, lower respiratory sources, pleural/chest fluid, pericardial fluid, and wound specimens

Testing Algorithm

See Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology in Special Instructions.

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma hominis DNA is not likely.

 

Submit only 1 of the following specimens:

 

Supplies: M5 media (T484), Culturette (BBL Culture Swab) (T092)

Specimen Type: Swab

Sources: Cervix, urethra, urogenital, vaginal, chest/mediastinal; bronchus (donor swab); or upper respiratory sources (only infants <3 months: nasopharynx, nose, throat)

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium: T092)

Acceptable: Swab in transport media: M4, M5 (T484), M6, universal transport media, or ESwab

Specimen Volume: 1 swab

Collection Instructions:

Vaginal:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

Urethra or Cervical:

1. Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.

2. Place swab back into swab cylinder.

Wound:

1. Collect specimen by swabbing back and forth over wound surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

 

Supplies: M5 media (T484)

Specimen Type: Fluid

Sources: Pelvic fluid, amniotic fluid, prostatic secretions, semen, reproductive drainage or fluid, pleural/chest fluid, chest tube fluid, pericardial fluid, sputum, tracheal secretions, bronchial washings, bronchoalveolar lavage, lung; or nasal washings (only infants <3 months)

Container/Tube:

Preferred: Sterile container

Acceptable: Container with 3 mL of transport media: M4, M5 (T484), M6, or universal transport media

Specimen Volume: 1-2 mL

 

Specimen Type: Synovial Fluid

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Send specimen in original tube (preferred).

 

Specimen Type: Urine, kidney/bladder stone, or ureter

Container/Tube: Sterile container

Specimen Volume: 10 mL or entire specimen

 

Specimen Type: Tissue

Sources: Placenta, products of conception, respiratory, bronchus, chest/mediastinal, bone, or joint

Container/Tube: Sterile container

Specimen Volume: 5 mm(3)

Collection Instructions: Submit only fresh tissue.


Specimen Minimum Volume

Fluid: 1 mL
Urine: 2 mL
Swab: 1 swab
Tissue: 5 mm(3)

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, formalin-fixed and/or paraffin-embedded tissues, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge), anticoagulants other than EDTA, green top (heparin) tube, blue top (citrate) tube, yellow top (ACD) tube, serum separator tube (SST)

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MHRP Mycoplasma hominis PCR 68546-1

 

Result ID Test Result Name Result LOINC Value
SRC86 Specimen source 31208-2
32536 Mycoplasma hominis PCR 68546-1

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

Analytic Time

3 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.