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Test Code MISCLM Pernicious Anemia Cascade

Additional Codes

 

MAYO Test ID

ACASM

EPIC Test ID

2608

 

Reporting Name

Pernicious Anemia Cascade

Useful For

Diagnosis of pernicious anemia

 

Diagnosis of vitamin B12 deficiency-associated neuropathy

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IFBPA Intrinsic Factor Blocking Ab, S Yes, (order IFBA) No
MMAPA Methylmalonic Acid, QN, S Yes, (order MMAS) No
GASTR Gastrin, S Yes, (order GAST) No

Testing Algorithm

If vitamin B12 is <150 ng/L, then intrinsic factor blocking antibody (IFBA) is performed. If IFBA is negative or indeterminate, then gastrin is performed.

If vitamin B12 is 150 to 400 ng/L, then methylmalonic acid (MMA) is performed. If MMA is >0.40 nmol/mL, then IFBA is performed.

 

See Vitamin B12 Deficiency Evaluation in Special Instructions.

Method Name

Immunoenzymatic Assay

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 4 mL

Collection Instructions:

1. Fasting (8 hours)

2. Divide specimen into 3 plastic vials, 1 containing 1 mL, 1 containing 1.5 mL, and 1 containing 1.5 mL

3. Band specimens together.

Additional Information: This test should not be ordered on patients who have received vitamin B12 injection within the last 2 weeks.


Specimen Minimum Volume

2.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen 14 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

NA

Special Instructions

Reference Values

180-914 ng/L

Day(s) and Time(s) Performed

Monday through Friday 5 a.m.-12 a.m.

Saturday 6 a.m.-6 p.m.

CPT Code Information

82607-Vitamin B12 assay

82941-Gastrin (if appropriate)

83921-MMA (if appropriate)

86340-IFBA (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ACASM Pernicious Anemia Cascade 2132-9

 

Result ID Test Result Name Result LOINC Value
B12PA Vitamin B12 Assay, S 2132-9

Analytic Time

Same day/1 day

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)