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Test Code MISCLM BRAF Mutation Analysis (V600E), Tumor

Additional Codes

MAYO Test ID
BRAFT
EPIC Test ID
LAB2608

 

Useful For

An adjunct to MSIHC / Microsatellite Instability (MSI)/Immunohistochemistry (IHC) Profile-Lynch/Hereditary Nonpolyposis Colorectal Cancer (HNPCC) Screen, when colon tumor demonstrates microsatellite instability (MSI-H) and loss of MLH1 protein expression, to help distinguish a somatic versus germline event prior to performing expensive germline testing

 

An adjunct to negative MLH1 germline testing in cases where colon tumor demonstrates MSI-H and loss of MLH1 protein expression

 

Note: Mayo's preferred screening test (BRMLH / MLH1 Hypermethylation and BRAF Mutation Analyses, Tumor) includes both MLH1 promoter hypermethylation and BRAF V600E testing.

 

To identify colon tumors with a previously negative KRAS mutation analysis result that may respond to epidermal growth factor receptor-targeted therapies.

 

To identify melanoma tumors that may respond to anti-BRAF targeted therapies.

Note: This is a laboratory developed test and has not been FDA-approved for this purpose.

Additional Tests

Test ID Reporting Name Available Separately Always Performed
SLIRV Slide Review in MG No, (Bill Only) Yes

Testing Algorithm

When this test is ordered, slide review will always be performed at an additional charge.

 

See Hereditary Nonpolyposis Colorectal Cancer Testing Algorithm in Special Instructions.

Method Name

Polymerase Chain Reaction (PCR) Analysis

Reporting Name

BRAF Mutation Analysis(V600E), Tumor

Specimen Type

Varies


Necessary Information


Pathology report must accompany specimen in order for testing to be performed.



Specimen Required


Preferred

Specimen Type: Formalin-fixed, paraffin-embedded tissue block

 

Acceptable

Specimen Type: Tissue

Slides: 1 stained and 10 unstained

Collection Instructions: Submit 1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides with 5-micron thick sections of the tumor tissue.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Frozen 
  Refrigerated 

Reject Due To

No specimen should be rejected.

Reference Values

An interpretative report will be provided.

Day(s) and Time(s) Performed

Monday, Wednesday, Friday; 10 a.m.

Analytic Time

14 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81210-BRAF (v-raf murine sarcoma viarl oncogene humolog B1) (eg, colon cancer), gene analysis, V600E variant

88381-Microdissection, manual

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BRAFT BRAF Mutation Analysis(V600E), Tumor In Process

 

Result ID Test Result Name Result LOINC Value
53217 Result Summary 50397-9
53218 Result 58415-1
53219 Interpretation 69047-9
53220 Specimen 31208-2
53221 Source 31208-2
54439 Tissue ID No LOINC Needed
53222 Released By No LOINC Needed

Forms

1. Molecular Genetics: Inherited Cancer Syndromes Patient Information (T519) in Special Instructions.

2. If not ordering electronically, complete, print, and send an Oncology Test Request Form (T729) with the specimen

(http://www.mayomedicallaboratories.com/it-mmfiles/oncology-request-form.pdf)