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Test Code MISCLM Insulin, Free and Total, Serum

Additional Codes



Useful For

Assessing free (bioactive) insulin concentrations in patients with known or suspected insulin antibodies

Profile Information

Test ID Reporting Name Available Separately Always Performed
INSF Insulin, Free, S No Yes
INSTO Insulin, Total, S Yes, (Order as INS) Yes

Method Name

Electrochemiluminescence Immunoassay

Reporting Name

Insulin, Free and Total, S

Specimen Type


Specimen Required

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Fasting (8 hours)

2. Avoid hemolysis

3. Label specimens with corresponding draw times.

4. Serum-gel tubes should be centrifuged within 2 hours of collection.

5. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

6. Send frozen to laboratory

Additional Information: If multiple specimens are drawn, send separate order for each specimen.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 180 days
  Refrigerated  7 days

Reject Due To


Mild reject; Gross reject


Mild OK; Gross OK




Autopsy/ postmortem specimen

Reference Values

Insulin, Free, S

2.6-24.9 mcIU/mL


Insulin, Total, S

2.6-24.9 mcIU/mL


Day(s) and Time(s) Performed

Monday through Saturday; Continuously

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83527-Free Insulin

83525-Total Insulin

LOINC Code Information

Test ID Test Order Name Order LOINC Value
INSFT Insulin, Free and Total, S In Process


Result ID Test Result Name Result LOINC Value
INSTO Insulin, Total, S 20448-7
INSF Insulin, Free, S 6901-3