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Test Code MISCLM Vitamin B6 Profile (PLP and PA), Plasma

Additional Codes

 

MAYO Test ID

B6PRO

EPIC Test ID

LAB2608

 

Useful For

Determining the overall success of a vitamin B6 supplementation program

 

Diagnosis and evaluation of hypophosphatasia

Profile Information

Test ID Reporting Name Available Separately Always Performed
PLP Pyridoxal 5-Phosphate (PLP), P Yes Yes
B6PA Pyridoxic Acid (PA), P No Yes

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin B6 Profile (PLP and PA), P

Specimen Type

Plasma Heparin


Specimen Required


Patient Preparation:

1. Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).

2. Patient must not ingest vitamin supplements for 24 hours before the specimen is drawn.

Supplies: Amber Frosted Tube, 5 mL

Collection Container/Tube: Green top (sodium or lithium heparin)

Submission Container/Tube: Amber vial (T192)

Specimen Volume: 1 mL

Collection Instructions: Centrifuge at 4° C, then aliquot all plasma into amber vial to protect from light.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Heparin Refrigerated (preferred) 7 days
  Frozen  14 days

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

NA

Reference Values

PYRIDOXAL 5-PHOSPHATE

5-50 mcg/L

 

PYRIDOXIC ACID

3-30 mcg/L

Day(s) and Time(s) Performed

Monday, Thursday; 12:01 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

82542-Quantitative

84207-Pyridoxal phosphate (vitamin B6)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
B6PRO Vitamin B6 Profile (PLP and PA), P In Process

 

Result ID Test Result Name Result LOINC Value
61065 Pyridoxic Acid (PA), P 1688-1
4047 Pyridoxal 5-Phosphate (PLP), P 30552-4