Sign in →

Test Code MISCLM Chronic Lymphocytic Leukemia (CLL), FISH

Additional Codes

MAYO Test ID
CLLF
EPIC Test ID
LAB2608

 

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with chronic lymphocytic leukemia (CLL)

 

Identifying and tracking known chromosome abnormalities in patients with CLL and tracking response to therapy

 

Distinguishing patients with 11;14 translocations who have leukemic phase of mantle cell lymphoma from patients who have CLL 

 

Detecting patients with atypical CLL or other forms of lymphoma associated with translocations between IGH and BCL2, BCL3, MYC, or other partner genes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_PBCT Probe, +2 No, (Bill Only) No
_PADD Probe, +1 No, (Bill Only) No
_PB02 Probe, +2 No, (Bill Only) No
_PB03 Probe, +3 No, (Bill Only) No
_IL25 Interphases, <25 No, (Bill Only) No
_I099 Interphases, 25-99 No, (Bill Only) No
_I300 Interphases, ≥100 No, (Bill Only) No

Testing Algorithm

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred. See the Method Description for specific details.

 

Indicate if the entire panel is to be performed. The entire panel is suggested for patients with a new diagnosis of chronic lymphocytic leukemia (CLL). If the patient is being tracked for known abnormalities or disease progression, indicate which probes should be used.

 

Panel includes testing for the following abnormalities using the probes listed:

6q-, D6Z1/MYB

11q-, D11Z1/ATM

+12, D12Z3/MDM2

13q-, D13S319/LAMP1

17p-, TP53/D17Z1

t(11;14), CCND1/IGH

 

When an IGH rearrangement is identified, reflex testing will be performed to identify the translocation partner. Probes include identification of t(14;18)(q32;q21) IGH/BCL2 and t(14;19)(q32;q13) IGH/BCL3.

 

This assay detects chromosome abnormalities observed in the blood and bone marrow of patients with CLL. For testing paraffin-embedded tissue samples from patients with small lymphocytic lymphoma (SLL), order SLLF / Small Lymphocytic Lymphoma, FISH, Tissue.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

CLL, FISH

Specimen Type

Varies


Specimen Required


Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

 

Advise Express Mail or equivalent if not on courier service.

 

Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube: Green top (sodium heparin)

Specimen Volume: 7-10 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Other anticoagulants are not recommended and are harmful to the viability of the cells.

 

Specimen Type: Bone marrow

Container/Tube: Green top (sodium heparin)

Specimen Volume: 1-2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Other anticoagulants are not recommended and are harmful to the viability of the cells.


Specimen Minimum Volume

Blood: 2 mL/Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

No specimen should be rejected. If specimen not received at appropriate temperature or in wrong anticoagulant, include note to laboratory. Contact the laboratory with questions.

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.

Analytic Time

7 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88271x2, 88291 – DNA probe, each (first probe set), Interpretation and report

88271x2 – DNA probe, each; each additional probe set (if appropriate)

88271x1 – DNA probe, each; coverage for sets containing 3 probes (if appropriate)

88271x2 – DNA probe, each; coverage for sets containing 4 probes (if appropriate)

88271x3 – DNA probe, each; coverage for sets containing 5 probes (if appropriate)

88274 w/modifier 52 – Interphase in situ hybridization, <25 cells, each probe set (if appropriate)

88274 – Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)     

88275 – Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CLLF CLL, FISH In Process

 

Result ID Test Result Name Result LOINC Value
51906 Result Summary 50397-9
51908 Interpretation In Process
51907 Result Table No LOINC Needed
54547 Result No LOINC Needed
CG686 Reason For Referral 42349-1
CG687 Specimen 31208-2
51909 Source 31208-2
51910 Method 49549-9
55114 Additional Information 48767-8
53866 Disclaimer 62364-5
51911 Released by No LOINC Needed