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Test Code PCRMYC Mycoplasma pneumoniae, Molecular Detection, PCR

Additional Codes

 

MAYO Test ID

MPRP

EPIC Test ID

LAB261

 

Reporting Name

Mycoplasma pneumoniae PCR

Useful For

Diagnosis of infections due to Mycoplasma pneumoniae

Method Name

Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.

 



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma pneumoniae DNA is unlikely.

 

Specimen source is required.

 

Submit only 1 of the following specimens:

 

Supplies: M5 media (T484)

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum

Container/Tube:

Preferred: Sterile container

Acceptable: Specimen in M4, M5 (T484), M6, or UTM

Specimen Volume: 1 mL

 

Supplies: M5 media (T484), Culturette (BBL Culture Swab) (T092)

Specimen Type: Swab

Sources: Throat, nasal, or nasopharyngeal

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium: T092)

Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M5 (T484), M6, UTM, or ESwab

Specimen Volume: Swab

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

 

Specimen Type: Fluid

Sources: Pleural, pericardial, cerebrospinal

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL


Specimen Minimum Volume

Respiratory: 0.5 mL; Fluid: 0.5 mL; Swab: 1 swab

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge)

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Sunday

CPT Code Information

87581

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MPRP Mycoplasma pneumoniae PCR 29257-3

 

Result ID Test Result Name Result LOINC Value
SRCMP Specimen source 31208-2
62394 Mycoplasma pneumoniae PCR 29257-3

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Analytic Time

3 days

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)