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Test Code STLPCR Stool FilmArray PCR, LAB5043

Important Note

Useful for Rapid detection of gastrointestinal infections caused by:

- Campylobacter species (Campylobacter jejuni/Campylobacter coli/Campylobacter upsaliensis)

- Clostridium difficile toxin A/B

- Plesiomonas shigelloides

- Salmonella species

- Vibrio species (Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholera)

- Vibrio cholera

- Yersinia species

- Enteroaggregative E. coli (EAEC)

- Enteropatogenic E. coli (EPEC)

- Enterotoxigenic E. coli (ETEC)

- Shiga-like toxin-producing E. coli (STEC)

- E. coli 0157

- Shigella/Enteroinvasive E. coli (EIEC)

- Cryptosporidium species

- Cyclospora cayetanensis

- Entamoeba histolytica

- Giardia lamblia

- Adenovirus F 40/41

- Astrovirus

- Norovirus GI/GII

- Rotavirus A

- Sapovirus

 

Results of the panel are intended to aid in the diagnosis of illness and are meant to be used in conjunction with other clinical and epidemiological findings.

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements

Stool specimen preserved in Cary Blair/C&S transport media.

 

Collection instructions:

1.  Collect fresh stool specimen and submit 1 gram or 5 mL into the transport medium.

2.  Place stool in preservative within 2 hours of collection

3.  Label transport medium and order in computer.

4.  Transport to laboratory, ambient or refrigerated, within 4 days.

 

Note: The detection of microbial DNA or RNA is dependent upon proper sample collection, handling, transportation, storage, and preparation.  There is a risk of false-negative results due to the presence of strains with sequence variability or genetic rearrangements in the target regions of the assays.

 

The test is not recommended as a test of cure.

 

Repeat testing should not be performed on samples collected less than 7 days apart.

 

The presence of blood or mucous in the sample may interfere with testing.

Reference Ranges

Negative (for all targets)

 

Day (s) Test Set Up

Monday thru Sunday; continuously.

Methodology

Multiplex Polymerase Chain Reaction (PCR)

 

A negative result should not rule-out infection inpatients with a high pretest probability for gastrointestinal infection.  The assay does not test for all potential infectious agents of diarrheal disease.

 

Positive results do not distinguish between a viable/replicating organism and the presence of a nonviable organism or nucleic acid, nor do they exclude the potential for coinfection by organisms not contained within the panel.

 

 

Test Classification and CPT Coding

87507 - Gastrointestinal pathogen, includes multiplex amplified probe technique, multiple types or subtypes, 12-25 targets.