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Test Code CD4TM CD4 Count for Immune Monitoring, Blood

Additional Codes







Reporting Name

CD4 T-Cell Count

Useful For

Serial monitoring of CD4 T cell count in HIV-positive patients


Useful for follow-up and diagnostic evaluation of primary cellular immunodeficiencies, including severe combined immunodeficiency


T-cell immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized


Assessment of T-cell immune reconstitution post hematopoietic cell transplantation


Early screening of gross quantitative anomalies in T cells in infection or malignancies

Method Name

Flow Cytometry, Single Platform (CD3, CD4, CD8, CD45)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Whole Blood EDTA

Shipping Instructions

It is recommended that specimens arrive within 24 hours of draw. Draw and package specimen as close to shipping time as possible.

Necessary Information

Date of draw is required.

Specimen Required

Container/Tube: 4 mL Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions:

1. Send specimen in original tube. Do not aliquot.

2. For serial monitoring, we recommend that specimen draws be performed at the same time of day.

St. Lukes Hospital Cedar Rapids Note:

Specimen must arrive at Mayo and be tested within 72 hours from time of draw. Due to limited specimen stability, specimen must be received in the laboratory at St. Luke’s Hospital Cedar Rapids as soon as possible. Specimen will only be accepted Monday through Thursday and/or Friday until 1400. If a holiday occurs, the specimen must arrive no later than 1400 the day prior to the holiday. Please contact St. Luke’s reference laboratory prior to drawing the specimen around Thanksgiving, Christmas and New Year’s, due to limitations in courier service at these times.

Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Ambient 72 hours

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject




ACD or heparinized blood, specimen in aliquot tube

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) and Time(s) Performed

Monday through Sunday

CPT Code Information

86359-T cells, total count

86360-Absolute CD4/CD8 count with ratio

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCD4 CD4 T-Cell Count In Process


Result ID Test Result Name Result LOINC Value
3321 CD45 Total Lymph Count 27071-0
3316 % CD3 (T Cells) 8124-0
3322 CD3 (T Cells) 8122-4
3319 % CD4 (T Cells) 8123-2
3325 CD4 (T Cells) 24467-3
3320 % CD8 (T Cells) 8101-8
3326 CD8 (T Cells) 14135-8
3327 4/8 Ratio 54218-3
CMTTC Comment 69052-9

Analytic Time

Same day/1 day

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.