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Test Code HCVRTM Hepatitis C Virus (HCV) RNA Detection and Quantification by Real-Time Reverse Transcription-PCR (RT-PCR), Serum

Additional Codes







Advisory Information

For detection and quantification of HCV RNA in serum before, during, and after antiviral therapy for chronic hepatitis C.

Shipping Instructions

1. Freeze serum immediately, and ship specimen frozen on dry ice only.

2. If shipment will be delayed for more 24 hours, freeze serum at -20 to -80° C (up to 84 days) until shipment on dry ice.

Specimen Required

Supplies: Aliquot Tube, 5 mL (T465)

Collection Container/Tube: Serum gel

Submission Container/Tube: Polypropylene vial (T465)

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions.

2. Pour off serum into aliquot tube.

Useful For

Detection of acute hepatitis C virus (HCV) infection before the appearance of HCV antibodies in serum (ie, <2 months from exposure)


Detection and confirmation of chronic HCV infection


Quantification of HCV RNA in serum of patients with chronic HCV infection (HCV antibody-positive)


Monitoring disease progression in chronic HCV infection and response to antiviral therapy


Determining cure and detection of relapse after completion of antiviral therapy

Testing Algorithm

The following algorithms are available in Special Instructions:

-Hepatitis C: Testing Algorithm for Screening and Diagnosis

-Chronic Hepatitis C Treatment and Monitoring Algorithm: Interferon-Free Combination Therapy

Method Name

Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Reporting Name

HCV RNA Detect/Quant, S

Specimen Type

Serum SST

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum SST Frozen (preferred) 84 days
  Refrigerated  6 days

Reject Due To


Mild OK; Gross OK


Mild OK; Gross OK


Mild OK; Gross OK


Red-top tube or tube with any anticoagulant

Reference Values


Day(s) and Time(s) Performed

Monday through Saturday; 7 a.m.-4 p.m.

Analytic Time

Monday through Thursday, 1 day; Friday and Saturday, 3 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
HCVQN HCV RNA Detect/Quant, S 11011-4


Result ID Test Result Name Result LOINC Value
97291 HCV RNA Detect/Quant, S 11011-4


If not ordering electronically, complete, print, and send a General Request Form (T239) with the specimen (