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Test Code LHSV Herpes Simplex Virus (HSV), Molecular Detection, PCR

Important Note

If HSV by PCR is needed on CSF, please use Sunquest code SFPCR test CSF Film Array PCR.

In Epic Lab6330.

Additional Codes







Reporting Name

Herpes Simplex Virus PCR

Useful For

Aiding in the rapid diagnosis of herpes simplex virus (HSV) infections, including qualitative detection of HSV DNA in nonblood clinical specimens

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Necessary Information

Specimen source is required.

Specimen Required

Submit only 1 of the following specimens:


Specimen Type: Fluid

Sources: Pleural, peritoneal, ascites, pericardial, amniotic, or ocular

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.



BBL CultureSwab (T092)

M4-RT (T605)

Specimen Type: Swab

Sources: Genital, dermal, ocular, nasal, throat, or oral

Container/Tube: Multimicrobe media (M4-RT) (T605)

Specimen Volume: Entire collection

Collection Instructions: Place swab back into multimicrobe media (M4-RT [T605], M4, or M5)

Additional Information: Source information should include main anatomical site of collection. 


Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube: Sterile container

Specimen Volume: 1.5 mL


Supplies: M4-RT (T605)

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT [T605], M4, or M5)

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue.

Additional Information: Source information should include main anatomical site of collection.


Specimen Type: Urine (<1 month old infant)

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Specimen Minimum Volume

Body or Ocular Fluid: 0.3 mL; Respiratory Specimen: 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To








Calcium alginate-tipped swab, wood swab, or transport swab containing gel; formalin-fixed and/or paraffin-embedded tissues

Reference Values


Day(s) and Time(s) Performed

Monday through Saturday; Varies

CPT Code Information

87529 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LHSV Herpes Simplex Virus PCR 87431-3


Result ID Test Result Name Result LOINC Value
SS001 Specimen Source 31208-2
34797 HSV 1, PCR 16130-7
34798 HSV 2, PCR 16131-5

Analytic Time

1 day/same day

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (

General Request Form (T239) (