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Test Code MISCLM Lyme Disease, Molecular Detection, PCR

Additional Codes



Reporting Name

Lyme Disease PCR

Useful For

Supporting the diagnosis of Lyme disease in conjunction with serologic testing


Specific indications including testing skin biopsies when a rash lesion is not characteristic of erythema migrans, and testing synovial fluid or synovium to support the diagnosis of Lyme arthritis

Testing Algorithm

See Acute Tick-Borne Disease Testing Algorithm in Special Instructions.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Advisory Information

This assay does not detect Borrelia miyamotoi. If infection with this organism is suspected, order BMIYB / Borrelia miyamotoi Detection PCR, Blood or BMIYC / Borrelia miyamotoi Detection PCR, Spinal Fluid.

Necessary Information

Specimen source is required.

Specimen Required

Submit only 1 of the following specimens:


Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Label specimen as spinal fluid.


Specimen Type: Synovial fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Label specimen as synovial fluid.


Specimen Type: Tissue (fresh only)

Sources: Skin or synovial biopsy

Container/Tube: Sterile container with normal saline

Specimen Volume: Approximately 4 mm(3)

Collection Instructions:

1. Submit only fresh tissue.

2. Skin biopsies:

a. Wash biopsy site with an antiseptic soap. Thoroughly rinse area with sterile water. Do not use alcohol or iodine preparations. A local anesthetic may be used.

b. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.

3. Label specimen with source of tissue.

Specimen Minimum Volume

Spinal Fluid, Synovial Fluid: 0.3 mL; Tissue: NA

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To








Green-top (heparin) tube, yellow-top (ACD) tube, serum gel tube, blood, or plasma


Reference Values


Day(s) and Time(s) Performed

Monday through Saturday (June through November)

Monday through Friday (December through May)

CPT Code Information


87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PBORR Lyme Disease PCR In Process


Result ID Test Result Name Result LOINC Value
SRC71 Specimen Source 31208-2
23635 B. burgdorferi PCR In Process
38288 B. mayonii PCR 82474-8
38289 B. garinii/B. afzelii PCR 82472-2
38323 Lyme CSF Comment 59464-8

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Analytic Time

1 day


If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (