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Test Code MISCLM Mycoplasma genitalium, Molecular Detection, PCR

Additional Codes







Reporting Name

Mycoplasma genitalium PCR

Useful For

Rapid, sensitive, and specific identification of Mycoplasma genitalium from genitourinary and reproductive sources

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Necessary Information

Specimen source is required.

Specimen Required

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma genitalium DNA is not likely.


Submit only 1 of the following specimens:



M4-RT (T605)

Culturette (BBL Culture Swab) (T092)

Specimen Type: Swab

Sources: Cervix, urethra, urogenital, vaginal


Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium [T092])

Acceptable: Swab in transport media: M4, M4-RT (T605), M5, M6, universal transport medium, or ESwab

Specimen Volume: One swab

Collection Instructions:

1. Vaginal: Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Urethra or Cervical: Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.

3. Place swab back into swab cylinder.


Supplies: M4-RT (T605)

Specimen Type: Fluid

Sources: Amniotic, pelvic, prostatic secretion, reproductive drainage, semen


Preferred: Sterile container

Acceptable: Container with 3 mL of transport media: M4, M4-RT (T605), M5, M6 or universal transport media

Specimen Volume: 1-2 mL


Specimen Type: Urine, kidney/bladder stone, ureter

Container/Tube: Sterile container

Specimen Volume: 10 mL or entire specimen


Specimen Type: Tissue

Sources: Placenta, products of conception, genitourinary

Container/Tube: Sterile container

Specimen Volume: 5 mm

Collection Instructions: Submit only fresh tissue.

Specimen Minimum Volume

Pelvic Fluid, Amniotic Fluid, Prostatic Secretions, Semen, Reproductive Drainage or Fluid: 1 mL
Urine: 2 mL
Swab: 1 swab

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To








Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, formalin-fixed and/or paraffin-embedded tissues, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge)

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
MGRP Mycoplasma genitalium PCR 69935-5


Result ID Test Result Name Result LOINC Value
SRC88 Specimen source 31208-2
32532 Mycoplasma genitalium PCR 69935-5

Analytic Time

3 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.


If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (