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Test Code TSPOT T-Spot TB, LAB1196

Important Note

This test must be sent to reference lab the same day it is collected.  Therefore, specimen may only be collected between 4:00 a.m. and 4:00 p.m. Monday through Friday.  It must arrive at St. Luke's Lab by 5:00 p.m. 

This test cannot be drawn after 4:00 p.m. on Friday until 4:00 a.m. on Monday.  If a test needs to be collected during this time frame, contact the laboratory for additional instructions.

Performing Laboratory

Oxford Diagnositc Laboratories, Memphis, TN.

Specimen Requirements

Collection of the blood specimen utilizes a standard green-top collection tube (lithium heparin) provided by Oxford, collected during routine phlebotomy.  The volume required is as follows:

1.  Adults and children ≥10 years of age = 6 mL

2.  Children ≥2 and <10 years of age = 4 mL

3.  Children <2 years of age = 2 mL

Specimen is collected and transported to the laboratory as soon as possible.  Specimen needs to arrive at St. Luke's Lab by 5:00 p.m. Monday-Friday.  Send at ambient temperature and DO NOT SPIN.


NOTE: if you do not have the specific tube from Oxford, the test may still be collected in a sodium or lithium heparin tube that does not have a serum separtor, but you must have the appropriate volume.  So more than one tube may be required.


NOTE: If the patient is immunosuppressed, draw additional tubes to assure there are enough WBC's for testing.

Reference Values


Useful For

Used to aid in the diagnosis of Mycobacterium tuberculosis infection.  The T-Spot TB test is an indirect tset for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnistic evaluations.

Day(s) Test Set Up

Monday - Friday (needs to arrive in the laboratory by 5:00 p.m.).


The T-Spot TB test is an in-vitro diiagnositic test based on an enzyme-linked immunospot (ELISPOT) method.

Test Classification and CPT Coding

CPT 86481