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Test Code VONWIL von Willebrand Profile

Additional Codes







Reporting Name

von Willebrand Prof

Useful For

Detection of deficiency or abnormality of von Willebrand factor and related deficiency of factor VIII coagulant activity


Subtyping von Willebrand disease as type 1 (most common), type 2 variants (less common), or type 3 (rare)


This test is not useful for detection of hemophilia carriers.

Profile Information

Test ID Reporting Name Available Separately Always Performed
F8A Coag Factor VIII Activity Assay, P Yes Yes
VWAG von Willebrand Factor Ag, P Yes Yes
VWFX von Willebrand Factor Activity, P Yes Yes
CCC1 Interpretation No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IBETH Bethesda Units No No
F8IS Coag Factor VIII Assay Inhib Scrn,P No No
RIST Ristocetin Cofactor, P No No
CCC2 Special Coagulation Interpretation No No
VWFM von Willebrand Factor Multimer, P Yes, (order VWFM2) No

Testing Algorithm

Initial testing includes: coag factor VIII activity assay, von Willebrand factor (VWF) antigen, and VWF activity. 


If the factor VIII, VWF antigen, and VWF activity are normal, and VWF activity:VWF antigen ratio is greater or equal to 0.8, a computer-generated interpretive comment indicating no evidence of von Willebrand disease will be provided.


If VWF activity assay is less than 55%, VWF ristocetin cofactor activity assay will be performed. If VWF antigen is less than 55%, the VWF activity is less than 55%, or the VWF activity:VWF antigen ratio is less than 0.8, VWF multimer analysis will be performed.


If any test results are abnormal, all results will be reviewed by a coagulation consultant and a Special Coagulation Consultant Interpretation will be provided.


See von Willebrand Disease Profile in Special Instructions.

Method Name

F8A, F8IS, IBETH: Activated Partial Thromboplastin Time-Based Clotting Assay

VWAG: Automated Latex Immunoassay (LIA)

VWFX: Latex Particle Enhanced Immunoassay

RIST: Ristocetin Induced Aggregation of Washed Normal Platelets

VWFM: Agarose Gel Electrophoresis/Infrared Dye-Labeled Antibody Detection

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Plasma Na Cit

Specimen Required

See Coagulation Studies in Special Instructions.


Patient Preparation: Patient should not be receiving anticoagulant treatment (eg, heparin, Coumadin). If so, please note.


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vials

Specimen Volume: 3 mL in 3 plastic vials each containing 1 mL

Collection Instructions:

1. Specimen must be drawn prior to factor replacement therapy.

2. Spin down, remove plasma, and spin plasma again.

3. Freeze specimens immediately at ≤-40 degrees C, if possible.

4. Send specimens in the same shipping container.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. If priority specimen, mark request form, give reason, and request a call-back.

3. Each coagulation assay requested should have its own vial.

4. If multiple coagulation profiles are ordered, each profile must be on a separate order.

Specimen Minimum Volume

2 plastic vials each containing 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen 14 days

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject


Mild OK: Gross reject



Reference Values









Day(s) and Time(s) Performed

Monday through Friday; Varies

CPT Code Information

85240-Coagulation factor VIII assay

85246-von Willebrand factor antigen

85397-von Willebrand factor activity

85245-von Willebrand factor ristocetin cofactor activity (if appropriate)

85247-von Willebrand factor multimer (if appropriate)

85335-Bethesda titer (if appropriate)

85335-Coagulation factor VIII inhibitor screen (if appropriate)

85390-26-Special coagulation interpretation (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VWPR von Willebrand Prof 48593-8


Result ID Test Result Name Result LOINC Value
F8A Coag Factor VIII Activity Assay, P 3209-4
VWAG von Willebrand Factor Ag, P 27816-8
VWFX von Willebrand Factor Activity, P 68324-3
7525 Interpretation 69049-5
7769 Reviewed by: 18771-6

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.


1. Coagulation Patient Information (T675) in Special Instructions

2. If not ordering electronically, complete, print, and send a Coagulation Test Request Form (T753) with the specimen (

Analytic Time

Von Willebrand Profile (F8A, VWAG, VWFX): 2 days /Von Willebrand factor Multimer: 5-7 days