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Test Code VRESPM Viral Culture, Respiratory

Additional Codes







Reporting Name

Viral Culture, Respiratory

Useful For

Diagnosing viral infections in respiratory specimens

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
TISSR Tissue Processing No, (Bill Only) No
VID2 Additional Testing Virus Ident No, (Bill Only) No
SVIR Viral Smear, Shell Vial No, (Bill Only) No

Testing Algorithm

All routine viral cultures are inoculated into cell culture tubes for viral detection. The most common specimens received for routine testing include bronchoalveolar lavage, sputum, and throat. A rapid (16-hour incubation) shell vial cell culture assay will be inoculated when specimens are designated for herpes simplex virus or cytomegalovirus detection or as appropriate for source indicated.


If enterovirus or hand, foot, and mouth disease is suspected, clearly indicate "enterovirus" on request.

Method Name

Cell Culture

Shell Vial Assay for Herpes Simplex Virus or Cytomegalovirus

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Advisory Information

Source-based recommendation for testing:

Esophogeal specimens: for tissue, swabs, or brushings order VIRNR / Viral Culture, Non Respiratory


Infectious agent-based recommendations for testing:

If herpes simplex visus (HSV) is suspected in a neonatal patient (<1 month), order VHSV / Herpes Simplex Virus (HSV), Culture From Neonates


State Health Department testing only: specimens will not be accepted at Mayo Medical Laboratories for the following diseases (submit directly to your state health department).



-High-risk infectious agents (examples of high-risk infections agents include: Ebola and other causative agents of viral hemorrhagic fever, avian influenza, severe acute respiratory syndrome (SARS), and Middle Eastern respiratory syndrome coronavirus (MERS-CoV)

Shipping Instructions

Specimen must be transported at refrigerate temperature and swab specimens should be sent in viral transport media.


Specimens must be received and cultured in the laboratory within 7 days postcollection. 


Specimens for viral culture should be transported to the laboratory as soon as possible for optimal recovery.

Necessary Information

Specimen source is required.

Specimen Required

Specimen Type: Lower respiratory tract specimens such as bronchoalveolar lavage, bronchial washings or aspirates, tracheal aspirates or secretions, pleural fluid, nasal swab or washing or sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Nasopharynx


Nasopharyngeal Swab (Rayon Mini-Tip swab) (T515)

M4-RT (T605)

Specimen Volume: Entire collection

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab into M4-RT media (T605) or other viral transport media (M4 or M5).


Specimen Type: Throat


Culturette (BBL Culture Swab) (T092)

M4-RT (T605)

Swab, Sterile Polyester (T507)

Container/Tube: Multimicrobe Media (M4-RT) (T605)

Preferred: BBL CultureSwab (T092)

Acceptable: Dacron-tipped swab with plastic handle (T507)

Specimen Volume: Swab

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5).


Specimen Type: Tissue

Supplies: M4-RT (T605)

Sources: Lung and others
Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5).

Specimen Volume: Entire collection


Specimen Type: Oral


Swab, Sterile Polyester (T507)

M4-RT (T605)

Container/Tube: Dacron-tipped swab with plastic handle (T507)

Specimen Volume: Swab

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5).

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated 7 days

Reject Due To








Gel swab, E-swab, swab with wooden handle

Reference Values


If positive, virus is identified.

Day(s) and Time(s) Performed

Monday through Sunday; Varies

CPT Code Information

87252-Tissue culture inoculation

87176-Tissue processing (if appropriate)

87253-Additional testing virus, identification (if appropriate)

87254-Viral smear, shell vial (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VRESP Viral Culture, Respiratory 6584-7


Result ID Test Result Name Result LOINC Value
VRESP Viral Culture, Respiratory 6584-7

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Analytic Time

14 days


If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (