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Test Code TOPSUM Targeted Opioid Screen, Urine

Additional Codes







Useful For

This high-resolution accurate mass method qualitatively (present vs. not detected) identifies 33 opioid compounds (parent drug and metabolites) in urine to help determine compliance or identify illicit opioid drug use

Method Name

Liquid Chromatography-Tandem Mass Spectrometry, High Resolution Accurate Mass (LC-MS/MS HRAM)

Reporting Name

Targeted Opioid Screen, U

Specimen Type


Specimen Required

Supplies: Plastic 5-mL tube (T465)

Collection Container/Tube: Plastic urine container

Submission Container/Tube: Plastic, 5-mL tube (T465)

Specimen Volume: 3 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 14 days
  Frozen  28 days
  Ambient  72 hours

Reject Due To


Mild reject; Gross reject




Mild reject; Gross reject



Reference Values

Not Detected


Cutoff concentrations:

Codeine: 25 ng/mL

Codeine-6-beta-glucuronide: 100 ng/mL

Morphine: 25 ng/mL

Morphine-6-beta-glucuronide: 100 ng/mL

6-monoacetylmorphine: 25 ng/mL

Hydrocodone: 25 ng/mL

Norhydrocodone: 25 ng/mL

Dihydrocodeine: 25 ng/mL

Hydromorphone: 25 ng/mL

Hydromorphone-3-beta-glucuronide: 100 ng/mL

Oxycodone: 25 ng/mL

Noroxycodone: 25 ng/mL

Oxymorphone: 25 ng/mL

Oxymorphone-3-beta-glucuronide: 100 ng/mL

Noroxymorphone: 25 ng/mL

Fentanyl: 2 ng/mL

Norfentanyl: 2 ng/mL

Meperidine: 25 ng/mL

Normeperidine: 25 ng/mL

Naloxone: 25 ng/mL

Naloxone-3-beta-glucuronide: 100 ng/mL

Methadone: 25 ng/mL

EDDP: 25 ng/mL

Propoxyphene: 25 ng/mL

Norpropoxyphene: 25 ng/mL

Tramadol: 25 ng/mL

O-desmethyltramadol: 25 ng/mL

Tapentadol: 25 ng/mL

N-desmethyltapentadol: 50 ng/mL

Tapentadol-beta-glucuronide: 100 ng/mL

Buprenorphine: 5 ng/mL

Norbuprenorphine: 5 ng/mL

Norbuprenorphine glucuronide: 20 ng/mL

Day(s) and Time(s) Performed

Monday through Friday; Varies

Analytic Time

2 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

80364, G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TOPSU Targeted Opioid Screen, U In Process


Result ID Test Result Name Result LOINC Value
42323 Codeine In Process
42324 Codeine-6-beta-glucuronide In Process
42325 Morphine In Process
42326 Morphine-6-beta-glucuronide In Process
42327 6-monoacetylmorphine In Process
42328 Hydrocodone In Process
42329 Norhydrocodone In Process
42330 Dihydrocodeine In Process
42331 Hydromorphone In Process
42332 Hydromorphone-3-beta-glucuronide In Process
42333 Oxycodone In Process
42334 Noroxycodone In Process
42335 Oxymorphone In Process
42336 Oxymorphone-3-beta-glucuronide In Process
42337 Noroxymorphone In Process
42338 Fentanyl In Process
42339 Norfentanyl In Process
42340 Meperidine In Process
42341 Normeperidine In Process
42342 Naloxone In Process
42343 Naloxone-3-beta-glucuronide In Process
42344 Methadone In Process
42345 EDDP In Process
42346 Propoxyphene In Process
42347 Norpropoxyphene In Process
42348 Tramadol In Process
42349 O-desmethyltramadol In Process
42350 Tapentadol In Process
42351 N-desmethyltapentadol In Process
42352 Tapentadol-beta-glucuronide In Process
42353 Buprenorphine In Process
42354 Norbuprenorphine In Process
42355 Norbuprenorphine glucuronide In Process
65059 Opioid Interpretation 69050-3