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Test Code 25VITD Vit D 25 Hydroxy, LAB535

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements

Container/Tube:

Preferred: Mint green-top, lithium heparin tube

Acceptable:  Gold-top serum gel tube, Plain, red-top tube, lavendar-top, EDTA tube

Specimen Volume: 250 uL of serum

Stability:  8 hours at room temperature 20-25°C, 4 days at refrigerated temperature 2-8°C; otherwise they should be stored frozen -15 to -25°C up to 24 weeks.

Additional Information:

1. Grossly hemolyzed specimens should be recollected.

2.  No significant interference with biotin up to 600 ng/mL

Reference Values

Deficiency: ≤20 ng/mL

Insufficiency: 21-29 ng/mL

Preferred Level: ≥30 ng/mL

Possible Toxicity: >100 ng/mL

Day(s) Test Set Up

Monday through Sunday, 24/7

Useful For

25-Hydroxyvitamin D is the best indicator of vitamin D nutritional status and is helpful in the diagnosis of Vitamin D deficiencies.

Methodology

Chemiluminescence immunoassay for quantitative determination

Test Classification and CPT Coding

82306

Report Available

2 hours upon receipt in the lab