Test Code 25VITD Vit D 25 Hydroxy, LAB535
Performing Laboratory
St. Luke's Hospital Cedar Rapids
Specimen Requirements
Container/Tube:
Preferred: Gold-top serum gel tube(s)
Acceptable: Plain, red-top tube(s)
Specimen Volume: 250 uL of serum
Stability: If the assay is performed within 120 hours (5 days) of sample collection, the samples should be kept at 2-8°C; otherwise they should be stored frozen (-20°C or below).
Collection Instructions:
1. Fasting (preferred but not required).
2. Centrifuge immediately and separate from cells within 1 hour of draw.
3. Label specimen appropriately (serum).
4. Forward promptly.
Additional Information:
1. Grossly hemolyzed specimens should be recollected.
2. Lipemic samples should be ultracentrifuged before testing.
3. If there is a delay in transport of >5 days or if mailing time exceeds 5 days, spin down, remove serum from cells and freeze immediately in plastic vial. Send specimen frozen.
Reference Values
Severe deficiency: <10 ng/mL
Mild to moderate deficiency: 10-29 ng/mL
Optimum levels: 30-100 ng/mL
Toxicity possible: >100 ng/mL
Day(s) Test Set Up
Monday through Friday (days only)
Useful For
25-Hydroxyvitamin D is the best indicator of vitamin D nutritional status and is helpful in the diagnosis of Vitamin D deficiencies.
Methodology
Direct competitive chemiluminescence immunoassay (CLIA) for quantitative determination
Test Classification and CPT Coding
82306
Report Available
1-2 days upon receipt