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Test Code ABORH ABO/Rh, LAB895


Test Tube Method/Gel Method

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements


Preferred: Pink-top (EDTA) tube

Acceptable:  Lavender-top (EDTA) tube

Specimen Volume: 0.5 mL of EDTA whole blood

Stability: An EDTA, hand labeled, specimen drawn within 3 days of testing is the preferred specimen. 

              The sample should be tested as soon as possible following collection.  If a delay in testing

              occurs, the sample should be stored 2-6 C.  

Collection Instructions: If the specimen is to be used for transfusion purposes is must be

                                        correctly hand labeled from the armband with patient's full name,

                                        inpatient hospital medical record number or B-Band number,

                                       time/date collected and tech code/initials.


Reference Values

Not applicable

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding


86901-Rh (D)

Useful For

Useful in determining the blood type of patients.


ABO grouping and Rh typing are based on the principle of agglutination.  Normal human red blood cells possessing these antigens will agglutinate in the presence of antibody directed toward those antigens.


Routine testing for determining the ABO group consists of testing the red cells with anti-A and anti-B (forward type) and testing the plasma/serum with A1 and B red cells (reverse type).  Both the forward red cell testing and the reverse plasma testing are required for routine ABO tests on patients because each serves as a check on the other.