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Test Code DATG Direct Antiglobulin Test, LAB274


Anti-C3b, C3d: Test Tube

Anti-IgG: Gel Method

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements


Preferred: Pink-top (EDTA) tube

Acceptable: Lavender-top (EDTA) tube

Specimen Volume: 1 mL of EDTA whole blood

Stability: The sample should be tested as soon as possible following collection.  If a delay

               in testing occurs, the sample should be stored 2-6 C.  
Collection Instructions:

Note: Pregnancy, transfusion, and medication history may be required for interpretation.

Reference Values


Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding


Useful For

The direct antiglobulin test is used to demonstrate the presence or absence of immunoglobulin (IgG or complement) on the surface of red blood cells.  Cells that have immunoglobulin adsorbed to their surfaces are said to be sensitized.  The direct antiglobulin test shows whether or not red blood cells have become sensitized in vivo.