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Test Code FETBLD Fetal Bleed Screen, LAB896


Kleihauer-Betke Stain

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements


Preferred: Pink-top (EDTA) tube

Acceptable:  Lavender-top (EDTA) tube
Specimen Volume: 1 mL of EDTA whole blood

Stability: If there is a delay in testing, the specimen should be stored at 2-10 C,

                not stored for longer than 2 days, and testing completed so that Rh Immune

                Globulin can be administered within 72 hours.

Collection Instructions:
 Specimen should be drawn and testing performed as soon after suspected bleed as

          possible in non-delivered patients. If patient has delivered it is best to wait 1 hour after

          delivery of all products of conception to allow any fetal blood to mix thoroughly in the

          maternal circulation, but the sample should be collected as soon as possible thereafter.

Reference Values


Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding


Useful For

The fetal bleed screen is performed as part of a RHIGG workup.

In most cases the feto-maternal hemorrhage is not sufficient to cause a positive test, but in those cases where a significant volume of fetal blood has entered the maternal circulation, the test provides an indication that a quantitative test is required to determine whether the bleed was sufficient to warrant a larger dose of Rh-Immune Globulin to the mother.