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Test Code HBSAG Hepatitis B Surface Antigen (HBsAg), LAB471

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements

Container/Tube:

Preferred: Gold-top serum gel tube or plain, red-top tube

Acceptable: Green-top (lithium heparin or sodium heparin) tube or lavender-top (EDTA) tube 

Specimen Volume: 90 uL and dead space of serum or plasma 

Stability: Store samples at 2 to 8°C  for up to 14 days. Samples can be stored at 20-25 °C for 6 days. Samples on the analyzers should be analyzed within 2 hrs. Freeze samples, devoid of red blood cells, at or below -20°C for up to 6 months.  Thoroughly mix thawed samples and centrifuge at 10,000 g for 2 minutes before using. Samples can be frozen 6 times

Note:  This assay is affected by biotin interference.

 

Reference Values

Nonreactive

 

Reactive results are sent to Pathology Laboratory, UPH Des Moines

Clinical Testing | Pathology Laboratory

Day(s) Test Set Up

Monday-Sunday

Useful For

Hepatitis B surface antigen (HBsAg) is a distinctive serological marker of acute or chronic hepatitis B infection.  HBsAg is the first antigen to appear following infection with hepatitis B virus and is generally detected 1 to 10 weeks before the onset of clinical symptoms.  HBsAg assays are routinely used to diagnose suspected HBV infection and to monitor the status of infected individuals to determine whether the infection has resolved or the patient has become a chronic carrier of the virus.

Methodology

Sandwich Immunoassay Chemiluminometric

Test Classification and CPT Coding

87340

Report Available

Stat within 1 hr of receipt

Routine within 2 hrs of receipt