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Test Code HBSQAB Hepatitis B Surface Antibody, LAB3480



Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements


Preferred: Gold-top serum gel tube or plain, red-top tube

Acceptable: Green-top (lithium heparin or sodium heparin) tube or lavender-top (EDTA) tube 

Specimen Volume: 250 uL of serum or plasma

Stability: Specimen must be centrifuged within 24 hours.  Serum or plasma may be stored up to 7 days at 2-8C. Freeze samples, devoid of red blood cells, at or below -20°C for longer storage.  Thoroughly mix thawed samples and centrifuge at 10,000 g for 2 minutes before using.  Samples may be subjected to 4 freeze/thaw cycles.

Collection Instructions:

Note:  Individuals that have received blood component therapies, e.g. whole blood, plasma, immunoglobulin administered during the previous 3–6 months may have a false reactive anti-HBs result due to passive transfer of anti-HBs.

Reference Values

≥10 mIU/mL is indicative of Immunity to HBV

Day(s) Test Set Up

Monday through Friday (Day shift only)

Test Classification and CPT Coding


Useful For

The presence of antibody to hepatitis B surface antigen (anti-HBs) is used to determine immune status to HBV or disease progression in individuals infected with HBV.