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Test Code MISCLM Hemoglobin Variant, A2 and F Quantitation, Blood

Additional Codes

MAYO Test ID
HGBCE
EPIC Test ID
LAB2608

 


Advisory Information


This test is intended for monitoring purposes, such as the increase in hemoglobin F (Hb F) after therapy, or the levels of hemoglobin variants after transfusion. The HPFH / Hemoglobin F, Red Cell Distribution, Blood test is a flow cytometry assay that determines the distribution of Hb F within red blood cells.

 

If the patient has never been appropriately studied, hemoglobin electrophoresis is necessary (see HBELC / Hemoglobin Electrophoresis Cascade, Blood).



Necessary Information


 



Specimen Required


Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: ACD, heparin

Specimen Volume: 4 mL

Collection Instructions:

1. Submit fresh specimen.

2. Do not transfer blood to other containers.


Forms

1. Thalassemia/Hemoglobinopathy Patient Information (T358) in Special Instructions

2. If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Useful For

Monitoring patients with sickling disorders who have received hydroxyurea or transfusion therapy

Method Name

Capillary Electrophoresis

Reporting Name

Hb Variant, A2 and F Quantitation,B

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Refrigerated 10 days

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

NA

Icterus

NA

Other

NA

Reference Values

HEMOGLOBIN A

1-30 days: 5.9-77.2%

1-2 months: 7.9-92.4%

3-5 months: 54.7-97.1%

6-8 months: 80.0-98.0%

9-12 months: 86.2-98.0%

13-17 months: 88.8-98.0%

18-23 months: 90.4-98.0%

≥24 months: 95.8-98.0%

 

HEMOGLOBIN A2

1-30 days: 0.0-2.1%

1-2 months: 0.0-2.6%

3-5 months: 1.3-3.1%

≥6 months: 2.0-3.3%

 

HEMOGLOBIN F

1-30 days: 22.8-92.0%

1-2 months: 7.6-89.8%

3-5 months: 1.6-42.2%

6-8 months: 0.0-16.7%

9-12 months: 0.0-10.5%

13-17 months: 0.0-7.9%

18-23 months: 0.0-6.3%

≥24 months: 0.0-0.9%

 

VARIANT 1

0.0

 

VARIANT 2

0.0

 

VARIANT 3

0.0

Day(s) and Time(s) Performed

Monday through Saturday; 7 a.m. and 1 p.m.

Analytic Time

Same day/1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83020-Quantitation by Electrophoresis

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HGBCE Hb Variant, A2 and F Quantitation,B 43113-0

 

Result ID Test Result Name Result LOINC Value
41927 Hb A 20572-4
41928 Hb F 4576-5
41929 Hb A2 4551-8
41930 Variant 1 24469-9
41931 Variant 2 24469-9
41932 Variant 3 24469-9
41933 HGBCE Interpretation 78748-1