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Important Note

Please accompany specimen with Iowa Maternal Screen Test Request Form.


Can be performed without a 1st trimester nuchal translucency (NT) measurement, but a NT measurement is preferred.


Incorrect information, especially incorrect dating, will result in inaccurate risk assessment.  Requires nuchal translucency (NT) measurement by sonographer certified by FMF or NTQR.  The sonographer's name, certification number, and certifying organization must be on file with the laboratory to submit a NT measurement.  Call 319-335-4056 the first time a sonographer is submitting a NT measurement with a test request.  If NT is unobtainable, order Intergrated Screen.  The following information is required for test interpretation: Ultrasound date AND crown rump length (CRL) measurement, a NT measurement obtained between 10 weeks 0 days and 13 weeks 6 days is preferred, but not required, patient's date of birth, current weight, patient's race, if patient requires insulin, family history of NTD, and for IVF pregnancies, the age of the egg donor at time of egg retrieval.  This test cannot be performed on multiples.  Detection rates: 85% Down Syndrome 80% Trisomy 18 85% Neural Tube defects.  False positive Rate: 2.6 % without NT 1.9% with NT. Results are available after the second serum sample is processed.  If the second specimen is not received for sequential screening, the results are uninterpretable and no maternal risk will be provided.

Specimen Requirements

First sample of a 2-part test drawn between 10 weeks 0 days and 13 weeks 6 days gestation. Can be performed without a 1st trimester nuchal translucency (NT) measurement, but an NT measurement is preferred.  Results require a second sample between 15 weeks 0 days and 20 weeks 6 days gestation.  No risks are reported until Sample 2 is received and tested.


Container/Tube Type: Red top or Gold-top serum gel tube

Specimen: 1 mL serum

Transport Temperature:  Refrigerate and send with a cold pack

Stability: Refrigerate and specimen must be received within 9 days of collection

Collection Instructions: Dating by ultrasound crown rump length (CRL).  Serum sample drawn in the first trimester when the CRL is 32-80 mm. Incorrect dating will result in inaccurate risk assessment.  

Sample Minimum Volume

1 mL of serum drawn in BOTH first and second trimesters

Reference Values

Final interpretative report will be available when the second sample test results are complete, but a report stating the date range to collect the second sample will be issued.

Day (s) Test Set Up

Monday through Friday

Analytic Time

3-5 business days

Reject Due To


Samples not collected within the required gestation age range for the test requested.


Quantitative Chemiluminescent Immunoassay

Performing Laboratory

State Hygienic Laboratory (SHL) Coralville

CPT Coding

Charges incorporated in Integrated Sample 2