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Test Code PROGST Progesterone, Serum,LAB529

Important Note

Note for IVF providers:  DHEA supplementation will falsely increase the apparent concentration of progesterone in patients being treated with this drug.  Providers using progesterone levels as a criterion for fresh embryo transfer in patients supplemented with DHEA should assess progesterone levels using an assay that is not impacted by DHEA-S cross reactivity.  It is possible the LC-MS chromatographic assays for progesterone may not be impacted by DHEA-S.


Immunoenzymatic Chemiluminescent Assay

Performing Laboratory

St. Luke's Hospital Laboratory

Specimen Requirements

Do not use collection vials with gel separators due to time related adsorption.


Container/Tube: Plain, red-top tube(s)-Serum gel tube is acceptable only if serum is removed from the SST within 24 hours of collection.

Specimen: 0.5 mL of serum



  • Room temperature:  8 hours
  • Refrigeration (2°- 8°C):  48 hours
  • Frozen (≤ -20°C): Undetermined.  Freeze samples only once and mix thoroughly after thawing.

Transport Temperature: Refrigerated

Reference Values



0.28–1.22 ng/mL

Normal females


follicular phase

<1.40 ng/mL

luteal phase

3.34–25.56 ng/mL

mid luteal phase

4.44–28.03 ng/mL

Postmenopausal females

<0.73 ng/mL

Pregnant females

  • First Trimester
  • Second Trimester
  • Third Trimester


11.22–90.00 ng/mL

25.55–89.40 ng/mL

48.40–422.50 ng/mL


Day(s) Test Set Up

Tuesday and Friday

STAT testing available Monday through Friday, day shift only

Test Classification and CPT Coding


Useful For

Progesterone levels are low during the follicular phase of the menstrual cycle. After ovulation, progesterone production by the corpus luteum increases rapidly, reaching a maximum concentration 4 to 7 days after ovulation. These levels are maintained for 4 to 6 days then fall to baseline levels, inducing menstruation.  During pregnancy, progesterone levels rise steadily to their highest levels in the third trimester. Clinical evaluation of progesterone confirms ovulation and normal luteal function in nonpregnant women. Inadequate progesterone production by the corpus luteum may indicate luteal phase deficiency (LPD), which is associated with infertility and early miscarriage.