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HelpTest Code PTHIN PTH, Intact, LAB108
Performing Laboratory
St. Luke's Hospital Cedar Rapids
Specimen Requirements
If PTH with minerals is needed, EDTA plasma can not be used for the mineral (calcium, phosphorus, creatinine) portion.
Container/Tube:
Preferred: Lavender-top (EDTA) tube
Acceptable: Plain, Red top serum
Minimum Volume: 30 uL plus dead volume
Stability: Because of the instability of PTH in unseparated serum, serum tubes should be centrifuged immediately. In contrast, PTH was found to be stable for 24 hours at room temperature in whole blood anticoagulated with EDTA.
Do not use Hemolyzed samples.
Patient sample stability is outlined in the following table:
|
Temperature |
Whole Blood |
EDTA Plasma |
Serum |
|
15‑25 °C |
24 hours |
48 hours |
8 hours |
|
2‑8 °C |
24 hours |
72 hours |
48 hours |
|
‑20 °C (± 5 °C) Freeze only once |
Unacceptable |
6 months |
6 months |
Reference Values
15.0 - 65.0 pg/mL
Day(s) Test Set Up
Monday through Sunday
Useful For
Measurement of intact PTH is useful in differentiating between hypercalcemia due to hyperparathyroidism and hypercalcemia of malignancy. However, the assay is not intended as and should not be relied upon as a diagnostic indicator of malignancy.
Methodology
Chemiluminescence Sandwich Assay
Test Classification and CPT Coding
83970
Report Available
STAT within 1 hour upon receipt
Routine within 2 hours upon receipt