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Test Code SCBPRT Culture Blood Port Line, LAB2172


Fluorometric Detection of CO2

CO2 indicates growth in broth culture bottle.

Note: This test is performed if physician is concerned that a central venous line is colonized or infected.

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements

1. Contact microbiology laboratory for instructions on specimen collection and to obtain special collection bottles.
2. 20 mL (10 mL in aerobic bottle and 10 mL in anaerobic bottle) of whole blood drawn from line following stoppage of IV (no waste is drawn).
3. Maintain sterility and forward promptly.

Note: 1. To avoid contamination, blood culture collection protocol must be followed.
2. Indicate specimen source/port on specimen container and in computer.

Reference Values

No growth

Critical value: all positives


If blood culture is positive, PCR is performed, if appropriate.

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding



87150 x 12 (PCR testing)