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Test Code COR30M Cortisol Stimulation 30 minutes, Lab2886

Important Note

Cortisol Baseline must also be ordered as a part of this test.  To perform a 30-minute stimulation test, order a baseline cortisol and also order the 30-minute stimulation test.


If both 30 and 60 minute levels are to be done, order one baseline, one 30-minute, and one 60-minute test.


If the test requested is for a cortisol only, order the cortisol a.m. or cortisol p.m. tests, as applicable.


This test is not recommended for pregnant or nursing women.




Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements

It is recommended that procedure be started between 0700 and 0900. Cortrosyn is available from the pharmacy or Pyxis.


Baseline - REQUIRED (30-minute post dose specimen must also be submitted.)



Submit specimen according to guidelines in procedure "Cortisol Stimulation Baseline, LAB2385)


30-Minute Post Dose

Submit only 1 of the following specimens:




Preferred: Green-top (lithium heparin) tube

Acceptable: Gold-top serum gel tube or Plain Red-top serum tube
Specimen: 170 uL of plasma or serum

Stability: Keep tubes stoppered and upright at all times.  Do not use samples that have been stored at room temperature for longer than 8 hours.  Tightly cap and refrigerate specimens at 2° to 8°C if the assay is not completed within 8 hours.  Freeze samples at or below -20°C if the sample is not assayed within 48 hours.  Freeze samples only once and mix thoroughly after thawing.

Collection Instructions: Draw post dose specimen 30 minutes after a 0.25 mg dose of Cortrosyn is injected IM.

Note: Label specimen appropriately (30 minutes post Cortrosyn injection).



Reference Values

Baseline (AM): 4.3-22.4 mcg/dL

Cortisol 30 min:  18-20 mcg/dL

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding


Useful For

The ACTH stimulation test is useful in the screening of patients presumed to have adrenocortical insufficiency.