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Test Code FVIII Factor 8 Assay, LAB306

Performing Laboratory

UPH Pathology Laboratory, Des Moines

Clinical Testing | Pathology Laboratory

Specimen Requirements

Container/Tube:

Preferred:Full blue-top (sodium citrate) 1.8mL tube-Hemolyzed or short draw specimen is not acceptable.

Specimen Volume:  0.5mL sodium citrate plasma
Stability: Platelet poor plasma should be frozen.

Collection Instructions: Forward promptly. If there is a delay in transport of >4 hours centrifuge specimens, remove plasma from cells, centrifuge aliquot for platelet poor plasma, transfer plasma from second spin into labeled aliquot without disturbing the bottom layer. Freeze immediately in plastic vial. Send specimen frozen. Specimen stability is ≤2 weeks when frozen at -20° C.

NOTE: Patients with a hematocrit of >55% must be recollected using an altered amount of sodium citrate

Reference Values

50 - 150% activity

Day(s) Test Set Up

Monday through Friday (days only)

Useful For

Hemophilia A is a sex-linked hemorrhagic disease caused by reduced circulating Factor VIII.  In patients with the disease, the degree of Factor VIII deficiency dictates the severity of the bleeding disorder.  Approximately 10-40% of normal Factor VIII activity is required for minimal hemostasis; below this range a tendency toward bleeding is apparent.

Methodology

Activated Partial Thromboplastin Clot Based Assay

Test Classification and CPT Coding

85240

Report Available

1 day upon receipt