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Polymerase Chain Reaction (PCR)/DNA Extraction/PCR Amplification/Hybridization

Used for the detection of SA/MRSA in wound specimens.

Note: 1. If specimen source is nasal for pre-surgical screening for MRSA/Methicillin-sensitive Staphylococcus aureus (MSSA); order #SMRSUR Methicillin-Resistant Staphylococcus aureus (MRSA) Detection by PCR, Presurgical. If specimen source is nasal for routine screening, order #SPMRSA Methicillin-Resistant Staphylococcus aureus (MRSA) Detection by PCR.

2. If MRSA is detected, infection control will be notified and inpatients will be isolated.

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements

Acceptable Specimens:

Wound screening for colonization or pre-surgical screening


Unacceptable Specimens:

Presurgical nasal screening or routine nasal screening


1. Submit specimen using aerobic CultureSwab.

2. The CultureSwab contains 2 swabs; using both swabs, culture the wound to be screened and place both swabs in transport media.

3. Maintain sterility and forward promptly.

Note: Indicate specimen source on specimen container and in computer.

Reference Values

No SA/MRSA isolated

Critical value: all positives

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

87640-Staphylococcus aureus

87641-Staphylococcus aureus, methicillin resistant